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KMID : 1005220080230020077
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Journal of Embryo Transfer
2008 Volume.23 No. 2 p.77 ~ p.80
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Effects of Cryoprotectants on In Vitro Development of Vitrified Immature Porcine Oocytes Following ICSI
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Lee Bong-Gu
Rhee Man-Hee
Kim Sang-Keun
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Abstract
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In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrified-thawed porcine oocytes were examined. Also, the developmental capacity of vitrified-thawed immature porcine oocytes following ICSI was investigated. Oocytes were cultured in NCSU-23 medium supplemented with 5% FBS at in 5% and air. The in vitro maturation rate of vitrified-thawed oocytes () was lower than that of the control (, p<0.05). The in vitro maturation rate of vitrified-thawed oocytes treated with CB + NCSU- 23 medium were , , , , respectively. The in vitro maturation rate () of the vitrified-thawed oocytes treated with CB for 30 min was the highest of all vitrification groups. When the in vitro developmental rates of the vitrified-thawed (with EDS and EDT) oocytes following ICSI were , , respectively. This results were lower than the control group ().
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KEYWORD
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porcine oocytes, vitrification, cytoclacin, in vitro developmental rates
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